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. 2021 Jun 23;22(2):897. doi: 10.3892/etm.2021.10329

Figure 3.

Figure 3

rhBMP-2 decreases the NLRP3 inflammasome activation and cell pyroptosis is induced by NLRP3-overexpression in AC16 cells. The expression of NLRP3, Caspase-1 and GSDMD-N was measured by (A) reverse transcription-quantitative polymerase chain reaction and (B) Western blotting. (C and D) The cell pyroptosis and (E and F) reactive oxygen species production were assessed using flow cytometry. (G) The concentrations of ANP, BNP, IL-1β and IL-18 were determined using ELISA. (H) The expression of NLRP3, Caspase-1 and GSDMD-N was measured using Western blotting. **P<0.01 and ***P<0.001, compared with the vector. #P<0.05, ##P<0.01 and ###P<0.001, compared with oeNLRP3. rhBMP-2, recombinant human BMP-2; NLRP3, NLR family pyrin domain-containing 3; NLRP3, NLR family pyrin domain-containing 3; GSDMD-N, gasdermin D; ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; IL, interleukin; oe, overexpression.