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. 2021 Jun 18;22(2):892. doi: 10.3892/etm.2021.10324

Figure 3.

Figure 3

SNHG11 is a sponge of miR-2355-5p in TNBC. (A) RNA-pull down assays assessed the potential binding between SNHG11 and the two predicated miRNAs. (B) The effect of SNHG11 silencing on the expression levels of two candidate miRNAs was analyzed with RT-qPCR. (C) RT-qPCR assessed miR-2355-5p expression in TNBC tissues and adjacent ones. (D) RT-qPCR confirmed overexpression efficiency of miR-2355-5p in BT-20 and BT-549 cells. (E) Putative binding fragment of SNHG11 and miR-2355-5p was predicted by starBase. (F) The interaction between SNHG11 and miR-2355-5p was confirmed via luciferase reporter experiments. (G) RIP assay validated the binding of SNHG11 and miR-2355-5p. (H) Spearman's correlation analysis verified the expression correlation between SNHG11 and miR-2355-5p in TNBC tissues. *P<0.05. SNHG11, small nucleolar RNA host gene 11; miR-2355-5p, microRNA-2355-5p; TNBC, triple-negative breast cancer; miRNAs, microRNAs; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; RIP, RNA immunoprecipitation; sh-, short hairpin; NC, negative control; Wt, wild-type; Mut mutant.