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. 2021 Jun 18;22(2):892. doi: 10.3892/etm.2021.10324

Figure 4.

Figure 4

CBX5 is a downstream of miR-2355-5p. (A) Seven potential target genes of miR-2355-5p through the starBase database are presented in the Venn diagram. (B) RT-qPCR examined the expression levels of candidate mRNAs after miR-2355-5p upregulation. (C) CBX5 expression was assessed with RT-qPCR in TNBC cells. (D) RT-qPCR determined CBX5 expression in TNBC tissues and adjacent ones. (E) Predicted sequence between CBX5 and miR-2355-5p was obtained from TargetScan. (F) The combination of CBX5 with miR-2355-5p was evaluated via luciferase reporter assay. (G) The binding ability of CBX5 and miR-2355-5p was verified through enrichment in Ago2-conjugated beads compared to in normal IgG via RIP assay. (H) The effect of SNHG11 knockdown or miR-2355-5p overexpression on CBX5 expression was confirmed by RT-qPCR and western blot analysis in TNBC cells. (I) Correlation between CBX5 and miR-2355-5p (SNHG11) expression in TNBC tissues was c with Spearman's correlation analysis. *P<0.05 and **P<0.01. CBX5, chromobox 5; miR-2355-5p, microRNA-2355-5p; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; TNBC, triple-negative breast cancer; RIP, RNA immunoprecipitation; SNHG11, small nucleolar RNA host gene 11; NC, negative control; sh-, short hairpin.