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. 2021 Jun 24;22(2):900. doi: 10.3892/etm.2021.10332

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Alignment of the first exon shown in all four versions of the NM_005348 mRNA of the HSP90AA1 gene. This exon has 138 nts in version 3 (NM_005348.3), which has been confirmed by a 5'-RACE assay, but it had only 60 nts in the first and second versions and has only 59 nts in the current version (NM_005348.4), due to the lack of the nt C (shaded) present in the three previous versions. The reverse transcription-PCR with the primer pair VF97 (underlined)/R1232 detected the band shown in Fig. 2; sequencing of the PCR product and a 5'-RACE assay confirmed the existence of the VF97 sequence therein. PCR, polymerase chain reaction; nt, nucleotide.