Table I.
HSF1 and HSP gene primers used.
| Gene | Primer name | Sequence | Primers/amplicon | Detecteda |
|---|---|---|---|---|
| HSF1 | HSF1F574 | 5'-ACAGCGTCACCAAGCTGCTG-3' | F574/R1312=739 bps | NM_005526.2; XM_005272315.1; XM_005272316.1; |
| HSF1R1312 | 5'-TTGTCCAGGCAGGCTACGCT-3' | XR_246618.2; XM_005272317.1 | ||
| HSF1XM17R | 5'-TGGCTGGACTTGGCCATGCG-3' | F574/RXM17R=642 bps | XM_005272317.1 | |
| HSF1R1576 | 5'-GTGTAGTGCACCAGCTGCTT-3' | F574/R1576=824 bps; 1003 bp | XR_246618.2; NM_005526.2 | |
| HSF1XR26 | 5'-TAGACATCTGTGGAGTGCGA-3' | F574/XR26=586 bps | XM_005272317.1 | |
| HSP90AA1 | HSP90F459 | 5'-AGGAAGCCCCTCTGAAGCCT-3' | F459/R1232=774 bps | NM_001017963.2; XM011536718.2 |
| HSP90R1232 | 5'-GTCCTCACTGTGAATGATCC-3' | |||
| HSP90VF97 | 5'-GTCGCTATATAAGGCAGGCG-3' | VF97/R1232=614 bps | NM_005348.3 | |
| HSPA1A | A1AF171 | 5'-CTTCTCGCGGATCCAGTGTT-3' | F171/R1104=934 bps | NM_005345.5 |
| A1AR1104 | 5'-CAGGGAGTCGATCTCCAGGC-3' | |||
| HSPA1B | A1BF146 | 5'-CTTGTCGCGGATCCCGTCCG-3' | F146/R1077=932 bps | NM_005346.4 |
| A1BR1077 | 5'-CAGGGAGTCGATCTCCAGGC-3' | |||
| HSPA6 | HSP6AF280 | 5'-GTGCGGAAAGGTTCGCGAAA-3' | F280/R1047=768 bps | NM_002155.3 |
| HSP6AR1047 | 5'-GAACCGACACATCGAAGGTG-3' | |||
| HSPA1L | A1L-F120 | 5'-GCTGCGTAATCTGGACGTTT-3' | F120/R899=780 bps | NM_005527.3; XM_005249070.2 |
| A1L-R899 | 5'-AGCCTGTTGTCAAAGTCCTC-3' | |||
| A1LV-F79 | 5'-GTGCAGTTTGATATTGAGGG-3' | VF79/R899=821 bps | NM_005527.3; XM_005249070.2 | |
| HSPB1 | HSPB1F19 | 5'-CTCAAACGGGTCATTGCCAT-3' | F19/R845=827 bps | NM_001540.3 |
| HSPB1R845 | 5'-CAAAAGAACACACAGGTGGC-3' | |||
| HSPB1R313 | 5'-AGTGTGCCGGATCTCCGAGA-3' | used in 5'-RACE | ||
| HSPB1F43 | 5'-AGAGACCTCAAACACCGCCT-3' | used in nested PCR | new variants | |
| HSPB1F70 | 5'-ATACCCGACTGGAGGAGCAT-3' | |||
| HSPB1R788 | 5'-ATCCGGGCTAAGGCTTTACT-3' |
aBecause the primers were designed and named years ago based on an earlier version of the RNA, the size of the amplicon and the number of the RNA version may or may not be the same as the current version in the NCBI database.