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. 2021 Jun 29;41(7):BSR20211066. doi: 10.1042/BSR20211066

Figure 2. Glucosamine increases the α-E catenin level in RAW 264.7 cells and glutamine is required for glucose-induced increase in α-E catenin.

Figure 2

RAW 264.7 cells were treated with indicated concentrations of glucose or glucosamine for 2 h after overnight serum starvation in the presence of 0.5 mM glucose. (A) Cell lysates were subjected to the Western blot analysis using α-E catenin and α-Tubulin antibodies. (B) Densitometry analysis of α-E catenin protein expression in Western blot image relative to the α-Tubulin. RAW 264.7 cells were treated with indicated concentrations of glucose in the presence or absence of glutamine for 2 h. (C) Cell lysates were subjected to the Western blot analysis using α-E catenin and α-Tubulin antibodies. (D) Densitometry analysis showing protein expression of α-E catenin normalised to α-Tubulin. Data represent mean ± S.E.M of at least four independent experiments. The unpaired t test was used to assess statistical significance, *P<0.05.