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. 2021 Jun 23;22(2):898. doi: 10.3892/etm.2021.10330

Figure 5.

Figure 5

CELF2 overexpression suppresses HUVEC tube formation by downregulating NFATc1. (A) Cell Counting Kit-8 assay was used to examine the viability of HUVECs transfected with OverExp-CELF2 and OverExp-NFATc1. *P<0.05 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆P<0.01 vs. OverExp-CELF2 + vector group. (B) HUVEC tube formation assay was used to examine the angiogenic ability of HUVECs transfected with OverExp-CELF2 and OverExp-NFATc1; scale bar, 100 µm. (C) Western blot analysis was used to examine the expression of proteins associated with angiogenesis and NFATc1 in transfected cells. *P<0.05 and ***P<0.001 vs. Con group. ###P<0.001 vs. OverExp-CELF2 group. ∆∆∆P<0.001 vs. OverExp-CELF2 + vector group. CELF2, CUGBP Elav-like family member 2; NFATc1, nuclear factor of activated T cells 1; Con, control; NC, negative control.