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. 2021 Jun 26;69:103450. doi: 10.1016/j.ebiom.2021.103450

Table 3.

a: MicroMethod, qPCR, serological and Discrete Typing Unit results in congenital Chagas Disease patients.

ID Site Umbilical Cord blood
 Peripheral Venous Blood
 Serodiagnosis at 10 months
 Discrete Typing Units
MM qPCR Par Load MM qPCR Par Load Age (days) Diagnosis ELISA HAI IFI SL IR II SL-IR I TaqMan PCR
1001 HC1 NA NA x Neg Neg x 64 Reactive 0·759 Ɛ 64 ¤ 32 ø Tc II/V/VI§ Neg Neg
1006 HC1 NA NA x Neg Neg x 118 Reactive 4·442§ 256 ¤ ND ND ND ND
1039 HC1 NA NA x Neg Pos 78·8 33 Discordant*◊ 0·015 ß 128 ¤ NR Tc II/V/VI Neg Neg
1044 HC1 NA NA x Pos Pos 3684 87 Non Reactive* 0·015 ß NR ¤ ND Tc II/V/VI Neg Tc V
1083 HC1 NA NA x Neg Pos 356 36 Reactive 2·985 ß 256 ¤ ND Tc II/V/VI Neg Neg
1111 HC1 NA NA x Pos Pos 641 10 Non Reactive* 0·036 ¥ NR ¤ ND Tc II/V/VI Neg Tc V
1167 HC1 NA NA x Pos Pos 37·5 49 Reactive 2·501 ß 256 ¤ ND Tc II/V/VI Neg Neg
2037 HC2 NA NA x Pos Pos 0 9 Non Reactive* 0·018 § NR ¤ ND ND ND ND
2047 HC2 NA NA x Pos Neg x 11 Reactive 2·624 § 256 ¤ ND Tc II/V/VI§ Neg Tc V
4045 HC3 Neg Pos 1418 Neg Pos 23709 54 Reactive 2·624 ¥ 256 ¤ ND Tc II/V/VI Neg Tc V
4055 HC3 Neg Neg x Neg Pos 53·4 41 Reactive 2·531 ß 128 ¤ ND Tc II/V/VI Neg Neg
4057 HC3 NA NA x Neg Pos 82·0 45 Reactive 1·418 ¥ 256 ¤ ND Tc II/V/VI Neg Neg
4058 HC3 Pos Pos 1370 Neg Neg x NA Non Reactive* 0·059 ¥ NR ¤ ND Tc II/V/VI Neg Tc V
6004 HC4 NA NA x Pos Neg x 1 Non Reactive* 0·017 § NR ¤ ND ND ND ND
b: MicroMethod, qPCR, serological and Discrete Typing Unit results in non congenital Chagas Disease patients.
1110 HC1 NA NA x Neg Pos 0·44 12 Non Reactive NR NR ¤ ND ND ND ND
4044 HC3 NA NA x Neg Pos 3·64 69 Non Reactive NR NR ¤ ND ND ND ND
4047 HC3 Neg Pos 39 NA NA x x Non Reactive NR NR ¤ ND Tc II/V/VI Tc I Neg
5069 HC5 Neg Pos 0·45 NA NA x x Non Reactive NR NR ¤ ND ND ND ND

MM: MicroMethod; Par load: Parasitic load in par.eq/mL * Treated patient: NA: Not available sample; ND: Not done; Pos: Positive; Neg: Negative; x: Not tested/No data; NR: Non Reactive

Ɛ cut-off: 0·152; § cut-off: 0·318; ß cut-off: 0·316; ¥ cut-off: 0·322; ¤ cut-off: dilution factor 16; ø cut-off: dilution factor 32; ◊ Case diagnosed by a positive MM on a second

routine control at 3 months of age and treated. § DTUs were identified in qPCR positive samples obtained at ten month of age.