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. 2021 Jun 26;69:103452. doi: 10.1016/j.ebiom.2021.103452

Figure 5.

Figure 5

ROCK inhibition interferes with YAP/TAZ in intestinal fibroblasts. (a) Representative images of immunohistochemical staining showing the expression of ROCK1 in different layers of stenotic and nonstenotic intestinal tissue from CD patients (n=10). (b) Western blot analysis of ROCK1 expression in three paired samples of fibroblasts derived from CD patients with and without stenosis. (c) The effects of the ROCK inhibitor Y27632 on the target genes of YAP/TAZ and α-SMA in intestinal fibroblasts isolated from stenotic regions of the intestine. (d) The effect of the ROCK inhibitor Y27632 on YAP/TAZ expression, YAP phosphorylation and α-SMA expression was time dependent. (e-f) Representative images of immunofluorescence staining showing the effect of the ROCK inhibitor on YAP and TAZ localization. Scale bar = 20 μm. In all cases, the bars in the graphs represent the mean ± S.D. Statistical analyses were performed using Student's t test and a chi-square test. Significant differences are shown by **p<0.01, ***p<0.001, and ****p<0.0001. All data shown are from three independent experiments.