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. Author manuscript; available in PMC: 2021 Jun 30.
Published in final edited form as: J Vis Exp. 2021 Mar 28;(169):10.3791/62447. doi: 10.3791/62447

Table 3: NTA results of monodisperse polystyrene bead standards.

Both standards were optimally diluted, with raw concentrations for the 100 nm standard and 400 nm standard of 4.205 × 107 particles/mL and 4.365 × 107 particles/mL, respectively. D10 is the point in the size distribution where 10% of the sample is contained, D50 is the point where 50% of the sample is contained (median), and D90 is the point where 90% of the sample is contained. SD: Standard deviation; CV: Coefficient of variation.

Size of Polystyrene Standard [nm] Integration range [nm] Mean size [nm] SD size [nm] CV of size D10 [nm] D50 [nm] D90 [nm] Total Counts
100 50 to 150 102 17 0.16 76 104 126 2628
400 300 to 500 391 47 0.12 150 358 456 2728