Table 4: NTA results of mouse perigonadal adipose tissue EVs demonstrate reproducibility of NTA measurements.

Raw NTA particle concentrations (particles/mL) measured on the particle tracking instrument and total particle concentration (particles/mL) adjusted for dilution factor of EVs derived from mouse perigonadal adipose tissue at various dilutions.

Dilution Factor	Particle Concentration, particles/mL	Total Particle Concentration, particles/mL
	Raw	Adjusted for Dilution Factor
1000	4.10E+07	4.10E+10
	4.00E+07	4.00E+10
	3.70E+07	3.70E+10
mean (SD)	3.93E+07 (1.70E+06)	3.93E+10 (1.70E+09)
1500	3.60E+07	5.40E+10
	2.60E+07	3.80E+10
	2.90E+07	4.40E+10
mean (SD)	3.03E+07 (4.19E+06)	4.55E+10 (6.60E+09)
2000	2.40E+07	4.80E+10
	2.30E+07	4.60E+10
	2.00E+07	4.00E+10
mean (SD)	2.23E+07 (1.70E+06)	4.46E+10 (3.40E+09)
3000	2.00E+07	6.00E+10
	1.30E+07	3.90E+10
	1.40E+07	4.20E+10
mean (SD)	1.57E+07 (3.09E+06)	4.71E+10 (9.27E+09)