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. Author manuscript; available in PMC: 2021 Jun 30.
Published in final edited form as: J Vis Exp. 2021 Mar 28;(169):10.3791/62447. doi: 10.3791/62447

Table 4: NTA results of mouse perigonadal adipose tissue EVs demonstrate reproducibility of NTA measurements.

Raw NTA particle concentrations (particles/mL) measured on the particle tracking instrument and total particle concentration (particles/mL) adjusted for dilution factor of EVs derived from mouse perigonadal adipose tissue at various dilutions.

Dilution Factor Particle Concentration, particles/mL Total Particle Concentration, particles/mL
Raw Adjusted for Dilution Factor
1000 4.10E+07 4.10E+10
4.00E+07 4.00E+10
3.70E+07 3.70E+10
mean (SD) 3.93E+07 (1.70E+06) 3.93E+10 (1.70E+09)
1500 3.60E+07 5.40E+10
2.60E+07 3.80E+10
2.90E+07 4.40E+10
mean (SD) 3.03E+07 (4.19E+06) 4.55E+10 (6.60E+09)
2000 2.40E+07 4.80E+10
2.30E+07 4.60E+10
2.00E+07 4.00E+10
mean (SD) 2.23E+07 (1.70E+06) 4.46E+10 (3.40E+09)
3000 2.00E+07 6.00E+10
1.30E+07 3.90E+10
1.40E+07 4.20E+10
mean (SD) 1.57E+07 (3.09E+06) 4.71E+10 (9.27E+09)