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. Author manuscript; available in PMC: 2021 Jun 30.
Published in final edited form as: J Vis Exp. 2021 Mar 28;(169):10.3791/62447. doi: 10.3791/62447

Table-of-Materials

Name of Material/ Equipment Company Catalog Number Comments/Description
1X dPBS VWR 02-0119-1000 To dilute samples
100 nm bead standard Thermo Scientific 3100A To test ViewSizer 3000 calibration
400 nm bead standard Thermo Scientific 3400A To test ViewSizer 3000 calibration
Centrifugal Filter Unit Amicon UFC901024 To filter PBS diluent
Collection tubes, 2 mL Qiagen 19201 For isolation of human plasma extracellular vesicles
Compressed air duster DustOff DPSJB-12 To clean cuvettes
Cuvette insert HORIBA Scientific - Provided with purchase of ViewSizer 3000
Cuvette jig HORIBA Scientific - To align magnetic stir bar while placing inserts inside cuvette; Provided with purchase of ViewSizer 3000
De-ionized water VWR 02-0201-1000 To clean cuvettes
Desktop computer with monitor, keyboard, mouse, and all necessary cables Dell - Provided with purchase of ViewSizer 3000
Ethanol (70-100%) Millipore Sigma - To clean cuvettes
ExoQuick ULTRA System Biosciences EQULTRA-20A-1 For isolation of human plasma extracellular vesicles
Glass scintillation vials with lids Thermo Scientific B780020 To clean cuvettes
“Hook” tool Excelta - Provided with purchase of ViewSizer 3000
Lint-free microfiber cloth Texwipe TX629 To clean cuvettes and cover work surface
Microcentrifuge tubes, 2 mL Eppendorf 22363344 For isolation of human plasma extracellular vesicles
Stir bar Sp Scienceware F37119-0005
Suprasil Quartz cuvette with cap Agilent Technologies AG1000-0544 Initially provided with purchase of ViewSizer 3000
ViewSizer 3000 HORIBA Scientific - Nanoparticle tracking instrument