Effects of 2-APB on nucleoplasmic and cytosolic Ca2+-transients (CaTs). A. Original recordings of [Ca2+]Nuc and [Ca2+]Cyto in one control (CTL) dog atrial cardiomyocyte (CM) before and after application of 50 μM 2-APB. B. Violin plots with median (solid line) ± interquartile range (dotted lines) values of diastolic [Ca2+] and CaT amplitude before and after 5, 25, 50 μM 2-APB in CTL CMs. C. Original recordings of [Ca2+]Nuc and [Ca2+]Cyto in one atrial-fibrillation (AF) dog atrial CM before and after application of 50 μM 2-APB. D. Mean±SEM values of diastolic [Ca2+] and CaT-amplitude before and after 5, 25, 50 μM 2-APB in AF CMs. P-values in black reflect pre-2APB vs. 2APB, P-values in blue italics reflect nucleus vs. cytosol based on repeated measures multilevel mixed effects models (n/N=CMs/dogs).