Figure 4.

A. Line-scan images and Ca²⁺-transients (CaTs) from single pixels at the regions indicated, along with median (solid line) ± interquartile range (dotted lines) violin plots for diastolic [Ca²⁺] and CaT-amplitude in cytosol and nucleus of 1-Hz (P1) and 3-Hz (P3) conditioned cardiomyocytes (CMs) with or without ITPR1 knockdown (KD), recorded at 1 Hz. B. Line-scan images and CaTs from single pixels at the regions indicated, along with mean±SEM violin plots for diastolic [Ca²⁺] and CaT-amplitude of cytosolic and nucleoplasmic CaTs in P1 and P3 CMs with or without ITPR2 KD, recorded at 1Hz. P-values in black reflect P1 vs P3, P-values in blue italics reflect with vs. without ITPR1/ITPR2 KD based on multilevel mixed effects models (n/N=CMs/dogs).