Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 29;21:750. doi: 10.1186/s12885-021-08495-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Addition of conditioned medium from OPNc overexpressed cells increased intracellular calcium levels in H1299 cultures. a Live fluorescence images for [Ca²⁺]i in H1299 cells transfected with GCaMP6s-tdTomato plasmid and stimulated with OPNc-CM, CM with addition of OPN-neutralizing antibody and integrin αvβ3-blocking antibody (ab78289). b Quantitative analyses for [Ca²⁺]i in H1299 cells transfected with GCaMP6s-tdTomato and stimulated with OPN-CMs. c Quantitative analyses for [Ca²⁺]i in H1299 cells stimulated with OPNc-CM, CM with addition of OPN-neutralizing antibody and integrin αvβ3-blocking antibody. A total of 10 to 30 individual cells were analyzed for ratios of GCaMP6s over tdTomato fluorescence intensities. Data were represented as the mean ± SD