Fig. 5.

Effects of OPNc on ROS levels in cisplatin treated H1299 cells by DCF fluorescent assays. a Flow cytometry from H1299 treated with 20 μg/ml cisplatin in OPN-c (with or without neutralizing antibody) CMs for 12 h. Quantification of the fluorescent intensities was shown as in the plot. b ROS levels in H1299 cells transfected with NFATc2 or mutants in 20 μg/ml cisplatin for 12 h. c Effect of OPNc-CM on ROS levels in NFATc2 transfected H1299 cells. Data were represented as the mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001