Table 2.

Experimental factors in coded and actual units and experimental responses.

Run	Independent variable in coded form				Independent variable in actual form				Glutamic acid (mg.mL−1)
	X1	X2	X3	X4	X1 (°C)	X2 (g.dL−1)	X3 (μg.L−1)	X4 (g.dL−1)	Actual	Predicted
1	-1	1	1	1	30	12	9	0.7	3.1	2.95
2	0	0	2	0	35	9	12	0.5	14.9	14.94
3	0	0	0	0	35	9	6	0.5	17.3	17.57
4	1	1	-1	-1	40	12	3	0.3	11.68	11.46
5	-1	-1	1	-1	30	9	9	0.3	19.9	19.84
6	-1	1	-1	1	30	12	3	0.7	3.8	3.67
7	1	-1	-1	-1	40	6	3	0.3	8.3	8.39
8	-1	-1	1	1	30	6	9	0.7	8.7	8.78
9	1	1	-1	1	40	12	3	0.7	9	9.09
10	0	0	0	2	35	9	6	0.9	2.78	3.01
11	2	0	0	0	45	9	6	0.5	6.65	6.75
12	0	0	0	-2	35	9	6	0.1	16.4	16.36
13	-1	-1	-1	1	30	6	3	0.7	8.7	8.57
14	0	0	-2	0	35	9	0	0.5	13	13.16
15	1	-1	1	1	40	6	9	0.7	8.1	8.11
16	1	1	1	1	40	12	9	0.7	8.28	8.08
17	1	-1	-1	1	40	6	3	0.7	8.4	8.18
18	-1	1	-1	-1	30	12	3	0.3	14.3	14.23
19	0	2	0	0	35	15	6	0.5	8.3	8.47
20	1	-1	1	-1	40	6	9	0.3	10.9	10.89
21	1	1	1	-1	40	12	9	0.3	12.96	13.03
22	-1	1	1	-1	30	12	9	0.3	16	16.08
23	0	0	0	0	35	9	6	0.5	17.5	17.57
24	0	0	0	0	35	9	6	0.5	17.9	17.57
25	-2	0	0	0	25	9	6	0.5	10.1	10.20
26	0	-2	0	0	35	3	6	0.5	11.2	11.23
27	-1	-1	-1	-1	30	6	3	0.3	16.9	16.97