Table 3.
Analysis of variance (ANOVA) for response surface quadratic model for Glutamic Acid production.
| Source | Sum of Squares | Df | Mean Square | F-value | p-value | |
|---|---|---|---|---|---|---|
| Model | 582.49 | 14 | 41.61 | 866.61 | <0.0001 | Significant |
| X1 (temperature) | 17.82 | 1 | 17.82 | 371.15 | <0.0001 | |
| X2 (glucose) | 11.45 | 1 | 11.45 | 238.57 | <0.0001 | |
| X3 (biotin) | 4.73 | 1 | 4.73 | 98.62 | <0.0001 | |
| X4 (urea) | 267.33 | 1 | 267.33 | 5568.24 | <0.0001 | |
| X1.X2 | 33.70 | 1 | 33.70 | 701.89 | <0.0001 | |
| X1.X3 | 0.0812 | 1 | 0.0812 | 1.69 | 0.2178 | |
| X1.X4 | 66.99 | 1 | 66.99 | 1395.41 | <0.0001 | |
| X2.X3 | 0.8742 | 1 | 0.8742 | 18.21 | 0.0011 | |
| X2.X4 | 4.69 | 1 | 4.69 | 97.63 | <0.0001 | |
| X3.X4 | 6.63 | 1 | 6.63 | 138.11 | <0.0001 | |
| X12 | 110.25 | 1 | 110.25 | 2296.41 | <0.0001 | |
| X22 | 79.43 | 1 | 79.43 | 1654.44 | <0.0001 | |
| X32 | 16.50 | 1 | 16.50 | 343.78 | <0.0001 | |
| X42 | 82.76 | 1 | 82.76 | 1723.74 | <0.0001 | |
| Residual | 0.5761 | 12 | 0.0480 | |||
| Lack of Fit | 0.3895 | 10 | 0.0389 | 0.4173 | 0.8588 | not significant |
| Pure Error | 0.1867 | 2 | 0.0933 | |||
| Correlation Total | 583.06 | 26 |