Table 5.
LPLA2 assay sensitivity, specificity, and accuracy in comparison to those based on the Ploemen criteria
| Sensitivity/Specificity | PPV/NPV | False Positives | False Negatives | Accuracy | |
|---|---|---|---|---|---|
| Ploemen model | 55/87 | 96.3/24 | atropine, 18 beta-glycyrrhetinic acid, S-methylisothiourea | azaperone, benzbromarone, bromocriptine, carbamazepine, clozapine, conessin, corticosterone, cyclopentolate, diclofenac, 5,7-dichloro-8-hydroxy-2-methyl quinolone, diphenhydramine, dutasteride, erythromycin, etomidate, fenofibrate, fipexide, flunarizine, flufenamic acid, fosinopril, fosinoprilat, fulvestrant, fusidic acid, gabapentin, gentisic acid, hydralazine, hydroxyzine, 6-hydroxydopamine, 3-hydroxytyramine, isoxsuprine, ketoconazole, ketotifen, lofepramine, loratadine, mannitol, mebhydrolin, melatonin, mianserin, mirtazapine, mitotane, oxolamine citrate, pantoprazole, penfluridol, perphenazine, phenacetin, phenytoin, pipamperone, pirenperone, pranlukast, progesterone, proparacaine, pyrilamine, repaglinide, ritanserin, spiperone, sulindac, sulpiride, suramin, tacrine, tetracaine, warfarin, yohimbine, zafirlukast | 58.2 |
| Modified Ploemen model | 76.4/95.7 | 99.1/40 | 18 beta-glycyrrhetinic acid | benzbromarone, carbamazepine, conessin, corticosterone, diclofenac, 5,7 dichloro-8-hydroxy-2-methyl quinolone, etomidate, fenofibrate, fipexide, flufenamic acid, fosinopril, fosinoprilat, fulvestrant, fusidic acid, gabapentin, gentisic acid, hydralazine, 6-hydroxydopamine, 3-hydroxytyramine, loratadine, mannitol, melatonin, mitotane, pantoprazole, phenacetin, phenytoin, pranlukast, progesterone, sulindac, sulpiride, suramin, warfarin, zafirlukast | 79.1 |
| LPLA2 (IC50 ≤ 500 μM) | 87.0/78.2 | 96/50 | carbamazepine, imiquimod, ritanserin, rolipram, ropinirole | azaperone, betaxolol, chloroquine (655), clenbuterol (7298), clindamycin, 5,7 dichloro-8-hydroxy-2-methyl quinolone (1179), disopyramide, dutasteride (1048), etomidate (1155), isoxsuprine, lidocaine, methapyrilene, phenacetin (2140), pranlukast, procyclidine (1470), proparacaine (2469), sulpiride, vinblastine (2123) | 85.9 |
| LPLA2 (IC50 any concentration) | 92.8/78.2 | 96.2/64 | carbamazepine, imiquimod, ritanserin, rolipram, ropinirole | azaperone, betaxolol, clindamycin, disopyramide, isoxsuprine, lidocaine, methapyrilene, pranlukast, sulpiride | 90.7 |
The table compares the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the Ploemen, modified Ploemen, and LPLA2 assays. Compounds associated with false-positive and false-negative results are listed. The numbers in parentheses denote the μM IC50 for LPLA2 inhibition.