Table-1.

Polymerase chain reaction master mix used for DNA amplification.

Component	Volume	Final concentration
GoTaq at Hot Start Green Master Mix. 2X	12.5 µL	1X
10 µM Primer F gene	2.5 µL	1.0 µM
10 µM Primer R gene	2.5 µL	1.0 µM
DNA ( ∼100 ng)	2 µL	200 ng
Nuclease-free water	Up to 25 µL	N.A
Total		25 µL

GoTaq® G2 Hot Start Green Master Mix Co., USA