Table-2.

Thermocycler conditions set for the polymerase chain reaction.

Step	Time	Temp	No.
Initial denaturation	3 min	95°C	1
Denaturation	15 s	95°C	35 cycles
Annealing	30 s	54°C
Polymerization	1 s	72°
Final extension	5 min	72°C	1
Infinity	----	4°C	----