REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken anti-GFP (green fluorescent protein)	Aves	Cat#GFP-1020 RRID:AB_10000240
Alexa Fluor® 488-AffiniPure F(ab'')2 Fragment Donkey Anti-Chicken IgY (IgG)	Jackson ImmunoResearch	Cat#703-546-155 RRID:AB_2340376
Rat anti-RFP (red fluorescent protein), clone 5F8	ChromoTek (Proteintech)	Cat#5F8-100 RRID:AB_2336064
Rabbit anti-Fibrillarin	Abcam	Cat#ab5821 RRID:AB_2105785
Rabbit anti-Histone H3, trimethyl (Lys 9)	Abcam	Cat#ab8898 RRID: AB_306848
Alexa Fluor™ 555 goat anti-rat IgG (H+L)	Thermo Fisher Scientific	Cat#A21434 RRID:AB_2535855
Goat anti-mouse IgG (H+L) Alexa Fluor™ Plus 488	Thermo Fisher Scientific	Cat#A32723 RRID:AB_2633275
Goat anti-mouse IgG (H+L) Alexa Fluor™ Plus 555	Thermo Fisher Scientific	Cat#A32727 RRID:AB_2633276
Goat anti-rabbit IgG (H+L) Alexa Fluor™ Plus 488	Thermo Fisher Scientific	Cat#A32731 RRID:AB_2633280
Goat anti-rabbit IgG (H+L) Alexa Fluor™ Plus 555	Thermo Fisher Scientific	Cat#A32732 RRID:AB_2633281
Goat anti-rabbit CF633	Biotium	Cat#20122 RRID:AB_10853773
Goat anti-mouse CF633	Biotium	Cat#20120 RRID:AB_10853296
Chemicals, peptides, and recombinant proteins
bisBenzimide H 33342 trihydrochloride (Hoechst 33342)	Millipore Sigma	Cat#B2261
PEI (Polyethylenimine)	Polysciences Inc.	Cat#23966
Paraformaldehyde powder, 95%	Millipore Sigma	Cat#158127
Triton™ X-100	Millipore Sigma	Cat#T8787
Tween-20	Millipore Sigma	Cat#P2287
Normal goat serum	Abcam	Cat#Ab7481 RRID:AB_2716553
PBS, 1× pH 7.4, without calcium and magnesium	Wisent	Cat#311-010-CL
Acryloyl-X (6-((acryloyl)amino)hexanoic acid)	Thermo Fisher Scientific	Cat#A20770
DMAA (N,N-dimethylacrylamide)	Millipore Sigma	Cat#274135
Sodium acrylate	Millipore Sigma	Cat#408220
Proteinase K	Millipore Sigma	Cat#P4850
Potassium persulfate	Millipore Sigma	Cat#216224
TEMED	Millipore Sigma	Cat#T7024
Experimental models:Cell lines
U-2 OS cells (human osteosarcoma epithelial; female derived)	ATCC	Cat#ATCC® HTB-96™ RRID:CVCL_0042
Recombinant DNA
pEGFP(C1)-RepoMan	Addgene	Cat#44212 RRID_Addgene44212
Software and algorithms
ZEN 3.0 (blue edition), Airyscan module	Zeiss	ZEN 3.0
Imaris Surfaces (3D Volume Rendering)	Oxford Instruments	IMARIS
Fiji/Image J image processing package (open source)	https://imagej.net/Fiji	FIJI
Other
μ-Slide 8-well chambered coverslip	ibidi	Cat#80826
μ-Slide 8-well chambered coverslip, glass bottom	ibidi	Cat#80827
Gold Seal Cover Glass, 18 × 18 mm, No. 1.5	Thermo Fisher Scientific	Cat#3405
Microscope cover glasses, 22 × 22 mm, No. 2	VWR	Cat#16004-304
N-EVAP Nitrogen Evaporator	Organomation	Cat#11106-O
Paragon sterile scalpels	Ultident	Cat#02-90000-24
Bemis™ Parafilm™ M Laboratory Wrapping Film	Fisher Scientific	Cat#ACAPM999
Sterile water, molecular grade	Wisent	Cat#809-115-CL
Petri dishes, 110 mm × 15 mm	Fisher Scientific	Cat#FB0875712
Cell culture dishes, non-treated, 35 mm	Millipore Sigma	Cat#EP0030700115