Fig. 4.

Comparative expression of cell type-specific marker genes in primary granule cell progenitors (GCPs) derived from P6 mice cerebellum and the immunostaining of BrdU-incorporated cells and apoptotic cells between WT and Jdp2-KO mice at P6. a Comparative expression of marker genes in the cerebellum between WT and Jdp2-KO mice. The granule cells were estimated by the expressions of Atoh-1, Zic1, Pax6, NeuroD1, and Lhx9; and Purkinje cells were marked by the expressions of Calbindin 1, 2, Doc2b and Pcp4. Bergman glia were estimated by expressions of Sept4 and Cdf10, basket cells are represented by Cck, and stellate cells by Gria2, astrocytes by Cd44, and oligodendrocytes by Oligo2. These expression patterns were calculated as total RNA expression and then summarized as a percentage of the relative RNA expression profiles in the cerebellum. The expression profiles of each cell type seem not to be big difference, but the expression of granule cells was higher in WT then Jdp2-KO mice. In contrast, the Purkinje cell expression profile in Jdp2-KO mice was higher than that in WT mice. b Comparative BrdU incorporation was evaluated by immunohistology of the cerebellum in WT and Jdp2-KO mice at P6. Expression of BrdU in cerebellum was 1.3-fold higher in WT than Jdp2-KO mice (80% vs 60%). Immunohistochemistry was performed as describe in the “Materials and methods” section. The section number was 10; the sample number was 5 mice for WT 129-C57/BL6J and 5 mice for Jdp2-KO 129-C57/BL6J mice; the lobes IV and V/VI were analyzed. *p < 0.05. c Expression of cytochrome C was examined by immunohistology of the cerebellum in WT and Jdp2-KO male mice at P6. Cytochrome C in cerebellum was higher in WT than Jdp2-KO mice. The section number was 14, and the sample number was 7 mice for WT 129-C57/BL6J and 7 mice for Jdp2-KO 129-C57/BL6J mice; the lobes IV and V/VI were analyzed. Immunohistochemistry was performed as describe in the “Materials and methods” section. **p < 0.01