Table 2.

The double-stranded biotinylated oligonucleotides for EMSA

Regulatory variants	Oligonucleotide sequences	Locations
g.31360 T>C (rs372004083)	5′-TTTTTACACGGTAA(T/C)AGGGGCCCTGTGATTG-3′	31346–31375
g.31436G>A	5′-CCCGCTGCGCTGGG(G/A)CCTCCAGGCTCTGAC-3′	31422–31451
g.31437C>A (rs769262495)	5′-CCGCTGCGCTGGGG(C/A)CTCCAGGCTCTGACG-3′	31423–31452
g.31487C>G	5′-GACACATTCCCCTC(C/G)CCCATACCCTGGAAG-3′	31473–31502
g.31856C>T	5′-AACTAGCATCCAGC(C/T)GGGCACCCCGGGTGA-3′	31842–31871

EMSA was performed with biotin-labeled oligonucleotide and H9c2 cell nuclear extracts. Free probe was indicated at the bottom. Solid arrows indicated the affected binding for unknown transcription factors

WT wild type, RV regulatory variant