Fig. 3.

Time dependent effects of OA on selected transcripts and E2 production. GCs were cultured under stimulatory conditions with FSH and IGF-1 supplementation throughout the time of culture. After 2 days, the cells were treated in addition with OA or vehicle control. Media with all respective supplements were replaced every other day. Transcript abundance of different genes (A, B, C, D, E, G) and levels of estradiol (F) were determined after 4, 6 and 8 days in culture in OA or vehicle treated GCs. OA treated samples showed reduced transcript abundance of CYP19A1, FSHR, LHCGR, CCND2, INHA FSHR, but increased levels of CD36. Different letters indicate significant differences of fold changes (mean fold change ± standard deviation, P < 0.05, one-way ANOVA from three independent experiments) relative to untreated, but FSH and IGF-1 stimulated cultures after day 4, which were set to one. Con denotes vehicle controls and OA denotes oleic acid treatment