Fig. 4.

Time dependent morphological changes of GCs after OA treatment. Following 2 days of stimulatory culture conditions with FSH and IGF-1 supplementation GCs were treated with OA or vehicle control in addition and photographed 6 h, 12 h, 24 h, 48 h, 4d and 6 later to study the OA induced changes of morphological features. A continuous change of the morphology from a fibroblast-like towards a flat, foam cell-like morphology can be seen. Cont denotes vehicle controls and OA denotes oleic acid treated cells. Scale bar = 50 μm