Table 4.

Effect of experimental diets on α-tocopherol, γ-tocopherol and pigments of liver from broilers

Item	Control	CH	CHR	CHM	SEM	p-value
Diterpene profile, µg/g
α-Tocopherol	6.66	6.07	5.43	4.91	0.626	0.199
γ-Tocopherol	1.26b	0.822a	0.833a	0.840a	0.070	< 0.0001
Pigments, µg/g
β-Carotene	0.977a	3.66c	3.15bc	2.11ab	0.388	< 0.0001
Chlorophyll-a1	2.44a	2.12a	3.66ab	4.21b	0.477	0.011
Chlorophyll-b2	4.94	3.36	6.12	5.68	0.897	0.161
Total chlorophylls3	7.33	5.49	9.78	9.67	1.38	0.102
Total carotenoids4	2.14a	10.1b	10.5b	8.49b	0.860	< 0.001
Total chlorophylls + Carotenoids5	9.48a	15.7b	20.2b	18.2b	1.65	< 0.001

The broilers were fed: (1) a corn-soybean based diet (Control); (2) the based diet plus 10 % C. vulgaris (CH); (3) diet 2 supplemented with 0.005 % Rovabio® Excel AP; and (4) diet 2 supplemented with 0.01 % of a pre-selected four-CAZyme mixture (CHM)

SEM - standard error of the mean

¹ Ca = 11.24 × A₆₆₂ − 2.04 × A₆₄₅

² Cb = 0.13 × A₆₄₅ − 4.19 × A₆₆₂

³ Ca + b = 7.05 × A₆₆₂ + 18.09 × A₆₄₅

⁴ Cx + c = (1000 × A₄₇₀ − 1.90 × Ca − 63.14 × Cb) /214

⁵ (Ca + b) + (Cx + c)

^a,b,c Mean values within a row with unlike superscript letters are significantly different (p < 0·05)