Figure 1.

A, Representative images of osteoclasts cultured in the presence or absence of heat‐aggregated IgG complexes, untreated and desialylated (de) during the last 24 hours of culture. Scale bar, 500 µm. B, TRAP positive cells with ≥ 3 nuclei were counted as osteoclasts. Results are shown as mean ± SEM, n = 8. Statistical analysis was performed using one‐way ANOVA followed by Tukey's multiple comparison test. MRL = macrophage colony‐stimulating factor + receptor activator of nuclear kappa‐B ligand (RANKL) stimulation