Fig. 2.

Biofilm assays of C. albicans, C. neoformans, and C. pelliculosa. Overnight cultures of C. albicans, C. neoformans, and C. pelliculosa were washed, diluted, and subcultured in Spider medium. The biofilm structures were developed by agitating on a rotor (100 rpm) at 37 °C for 48 h. Biofilm cells were then washed and stained using crystal violet, then quantified at 597 nm The 21 isolates of clinical C. pelliculosa and the references strains C. neoformans H99 and C. albicans SC5314 were tested. Five biological and five technical replicates were performed for each isolate