Fig. 4.

CircHIPK3 promoted MYH9 expression by binding to miR-124-3p. A Subcellular localization of circHIPK3 in chondrocytes was detected by nucleoplasmic separation experiment. B Binding sites between circHIPK3 and miR-124-3p were predicted by Bioinformatics website, and circHIPK3 wild plasmids (circHIPK3 wt) and circHIPK3 mutant plasmids (circHIPK3 mut) were constructed. C The results of dual luciferase reporter gene revealed that miR-124-3p bound to circHIPK3. D Bioinformatics website was searched to predict target genes that might bind to miR-124-3p (microT, PITA, PicTar, miRanda, Targetscan and miRmap). E Bioinformatics website was searched to predict binding sites of MYH9 to miR-124-3p, and construct MYH9 wild plasmids (MYH9 wt) and circHIPK3 mutant plasmids (MYH9 mut). F MiR-124-3p bound to MYH9 based on the dual luciferase reporter gene. G MRNA expression of MYH9 was detected by qRT-PCR after upregulation or suppression of miR-124-3p in chondrocytes. H Protein expression of MYH9 was detected by Western blot post upregulation or suppress of miR-124-3p in chondrocytes. *P < 0.05, **P < 0.01