Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 30;19:194. doi: 10.1186/s12951-021-00940-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — EVs-circHIPK3 derived from MSCs attenuated IL-1β induced chondrocyte injury. A Overexpressed circHIPK3 was observed in MSC EVs based on qRT-PCR. B With chondrocytes treated with IL-1β and MSCs-circHIPK3-EVs, the expression of miR-124-3p and MYH9 was detected by qRT-PCR. C MYH9 expression was detected through Western blot post treating chondrocytes with IL-1β and MSCs-circHIPK3-EVs. D With chondrocytes treated with IL-1β and MSCs-circHIPK3-EVs, the expression change in cartilage genes (Aggrecan, COL2A1, Runx2, Sox9 and MMP-13) was detected by qRT-PCR. E The expression change in key cartilage genes (Runx2, MMP-13, COL2A1, Aggrecan, and Sox9) was detected through Western blot after chondrocytes were treated with IL-1β and MSCs-circHIPK3-EVs. F Cell proliferation was detected through EdU experiment with chondrocytes treated with IL-1β and MSCs-circHIPK3-EVs. G Transwell experiment was conducted for the detection of cell proliferation after treating chondrocytes with IL-1β and MSCs-circHIPK3-EVs. H A flow cytometer was employed for the detection of cell apoptosis after chondrocytes were treated with IL-1β and MSCs-circHIPK3-EVs. *P < 0.05, **P < 0.01