Fig. 6.

MiR-124-3p overexpression reversed MSCs-circHIPK3-EVs mediated attenuation of chondrocyte injury. A The results of qRT-PCR demonstrated that miR-124-3p expression was considerately upregulated in chondrocytes transfected with miR-124-3p mimic. B–F MSCs-circHIPK3-EVs was used to co-culture chondrocytes transfected with miR-124-3p mimic, and the chondrocytes were pre-stimulated with IL-1β (10 ng/ml, 24 h). The expression of miR-124-3p and MYH9 was then detected through qRT-PCR (B); MYH9 expression was detected via Western blot (C); EdU experiment (D), Transwell experiment (E) and flow cytometry (F) were performed for the detection of cell proliferation, migration and apoptosis. *P < 0.05, **P < 0.01