Fig. 2.

Bioactive lipid mediators increase in cerebral cortex during developing amyloid pathology in App KI mouse model for AD. a, c AA-, b, d DHA- and e EPA-derived lipid mediators (LMs) were analyzed in the cerebral cortex and hippocampus of 2, 4, 8 and 18 months-old WT (n = 4–6) and App KI mice (n = 6–7) using LC–MS/MS. Horizontal bars indicate median. f Western blots for biosynthetic enzymes (COX-1, 15-LOX-1 and p-5-LOX) and g corresponding densitometric analysis in cerebral cortex and hippocampus of 2, 4, 8 and 18 months-old mice (n = 6/group). Comparisons between WT (blue) and App KI (red) mice were performed with the Mann–Whitney U test (^#P < 0.05, ^##P < 0.01, ^###P < 0.001). Kruskal–Wallis with Dunn’s post hoc test was used for multiple comparisons (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) AA = arachidonic acid, DHA = docosahexaenoic acid, EPA = eicosapentaenoic acid, COX-1 = cyclooxygenase-1, 15-LOX-1 = 15-lipoxygenase-1, p-5-LOX = phosphorylated (ser523) 5-lipoxygenase