Table 1.
Primary and secondary antibodies and reagents
| Protein targeted | Host | Dilution | Provider | Catalogue number |
|---|---|---|---|---|
| Primary antibodies | ||||
| Akt | Rabbit | 1:500 | Cell Signaling | 4691S |
| BLT1 | Rabbit | 1:400 | Cayman | 120114 |
| ChemR23 | Mouse | 1:500 | Santa Cruz | SC-398769 |
| COX-1 | Rabbit | 1:400 | R&D Systems | 4841S |
| c-PLA2 | Rabbit | 1:400 | Abcam | Ab73406 |
| ERK1/2 | Rabbit | 1:500 | Cell Signaling | 4696S |
| FPR2 | Rabbit | 1:500 | Santa Cruz | Sc-66901 |
| Galectin-3 | Goat | 1:200 | R&D Systems | AF1197 |
| GFAP | Rabbit | 1:800 | Dako | Z033401-2 |
| GPR18 | Rabbit | 1:400 | Sigma-Aldrich | SAB4501252 |
| Iba1 | Rabbit | 1:400 | Wako | 019-19741 |
| JUNK | Rabbit | 1:500 | Cell Signaling | 9252S |
| LGR6 | Rabbit | 1:500 | Invitrogen | PA5-102099 |
| MCSFR1 | Mouse | 1:500 | R&D Systems | MAB3291 |
| PLA1 | Rabbit | 1:500 | LSBio | LS-1310189 |
| p38 | Rabbit | 1:500 | Cell Signaling | 9212S |
| p-Akt | Mouse | 1:400 | Cell Signaling | 12694S |
| p-ERK1/2 | Mouse | 1:500 | Cell Signaling | 9106S |
| p-JUNK | Mouse | 1:500 | Cell Signaling | 9255S |
| p-cPLA2(S505) | Rabbit | 1:200 | Cell Signaling | 2831S |
| p-p38 | Mouse | 1:200 | Cell Signaling | 9216S |
| p-5-LOX | Rabbit | 1:400 | R&D Systems | pp2001 |
| S100β | Mouse | 1:400 | Sigma-Aldrich | AMAB91038 |
| TMEM119 | Rabbit | 1:400 | Thermo Fisher | PA5-62505 |
| Trem-2 | Sheep | 1:400 | R&D Systems | AF1729 |
| YKL-40 | Rat | 1:500 | R&D Systems | MAB2649 |
| 5-LOX | Rabbit | 1:500 | Abcam | Ab169755 |
| 15-LOX | Rabbit | 1:400 | Hans-Erik Claesson, KI | Ab154 |
| Aβ(1–16) peptide | Mouse | 1:500 | BioLegend | 803001 |
| Secondary antibodies and reagents | ||||
|
Alexa Fluor Plus 594 anti-mouse |
Donkey | 1:400 | Invitrogen | A32744 |
|
Alexa Fluor Plus 488 anti-rabbit |
Donkey | 1:400 | Invitrogen | A32790 |
|
IRDye®800CW anti-rabbit |
Donkey | 1:15,000 | LiCor | 926-32213 |
|
IRDye®680RD anti-mouse |
Donkey | 1:15,000 | LiCor | 926-68072 |
| IRDye®800CW anti-rat | Goat | 1:15,000 | LiCor | 926-32219 |
| IRDye®800CW anti-goat | Donkey | 1:15,000 | LiCor | 926-32214 |
|
Intercept® TBS Blocking buffer |
LiCor | 927-66003 |