Fig. 5.

Validation of a non-synonymous mutation in DIO2 gene and bioinformation analysis of DIO2 protein. A The position of the non-synonymous mutation in DIO2 gene. B Validation of the non-synonymous mutation in DIO2 gene in the population of S, W and L (left) and population of Tianfu meat geese breed (right). Black rectangle represented the mutant base in the population of S, W and L and population of Tianfu meat geese breed. S, Lion head goose; W, Sichuan White goose; L, Landes goose. “n” represented the population number of each breeds. C Homology comparison of DIO2 protein among different species. Consistent amino acids were shown in blue among different species. The Sec residues were shown in highlight red. Primary amino acid was shown in red, and mutant amino acid was shown in green. The number in the right represented the position of amino acid. D Phylogenetic tree analysis of DIO2 among different species. E Analysis of the secondary structure of DIO2 protein in knob- and non-knob geese