Figure 2.

Expression of IL15 and GZMB in the endometrium. (A) Histograms showing the distribution of the endometrial biopsy samples based on IL15 and GZMB gene expression levels. The distribution of the healthy controls is shown in blue and that of the samples from the patient group in red. A normal bell-shaped distribution is characteristic of the healthy control group (– – –), in contrast to the double-peaked contour that emerged when the patients’ samples were analyzed (——). The X-axis shows relative mRNA expression levels (IL15 or GZMB). The Y-axis shows the percent of samples with specified levels of IL15 or GZMB. (B-E) Endometrial biopsy samples with abnormal levels of IL15 (either low or high) were characterized by distinct molecular profiles. All samples were grouped into IL15 low, IL15 normal, and IL15 high samples according to the levels of IL15 transcripts in the sample. Filled triangles represent samples from the women with reproductive failures, open circles represent samples from the healthy controls. (B) The IL15 low samples revealed the lowest levels of GZMB and IL15RA. The IL15 high samples had the highest levels of GZMB and IL15RA. (C) The IL15 low samples had significantly lower levels of expression of FOXO1 and SLC2A1 in comparison with those of the IL15 normal and high samples. (D) Both the low and the high IL15 samples revealed decreased expression of SGK1 and SCNN1A. (E) The samples with high IL15 were marked with the lowest levels of PTGS2 and HEY1. ANOVA or Kruskal-Wallis test was used for statistical analysis between the IL15 low, normal, and high groups. ∗P<.05, ∗∗P<.01, ∗∗∗P<.001.