Fig. 2.

Focused 3D classifications on the PSI monomer map using differently placed and sized soft masks. The model in the centre depicts monomeric PSI bound to Cyt c₆ with mask locations on luminal (A, teal mask and B, magenta mask) and stroma sides (C, gold mask and D, light blue mask). The densities derived from 3D classifications of the symmetry expanded monomer dataset are shown colour coded by mask used and contoured at 5 sigma (mesh) and 20 sigma (surface). The class occupancy for each class of each focussed classification is given.