Table 1.
Binding affinities of protein-protein complexes with associated structural data.
| large Protein | small protein | organism | affinity (method) | structure |
|---|---|---|---|---|
| PSI | Flavodoxin | Synechococcus elongatus PCC 7942 | 0.02 μM (SPR) (Cao et al., 2020) | EM, 6KIF 3.3 Å (Cao et al., 2020)a |
| PSI | Ferredoxin | Thermosynechococcus elongatus BP-1 | 0.8 μM (FAS) (Kubota-Kawai et al., 2018)b | XRD, 5ZF0 4.2 Å (Kubota-Kawai et al., 2018)b |
| PSI | Plastocyanin | Chlamydomonas reinhardtii | 83 μM (enzymatic, FAS) (Sommer et al., 2002) | EM, EMDB-9853, not resolved (Suga et al., 2019) |
| PSI | Cyt c6 | Chlamydomonas reinhardtii | 116 μM (enzymatic, FAS) (Sommer et al., 2002) | no |
| bacterial reaction center |
Cyt c2 | Rhodobacter sphaeroides | 1 μM (FAS) (Moser and Dutton, 1988) | XRD, 1L9B 2.4 Å, 1L9J 3.2 Å (Axelrod et al., 2002) |
| bacterial reaction center |
Cyt c2 | Rhodobacter sphaeroides | 1 μM (FAS) (Moser and Dutton, 1988) | SANS (Tiede et al., 2000) |
| Cytochrome c Peroxidase |
Cyt c | Yeast | 0.01 μM–1 μM (ITC) (Erman and Vitello, 2002) | XRD, 2PCC 2.3 Å (Pelletier and Kraut, 1992) |
| Cytochrome c Peroxidase |
Cyt c | Yeast/Horse | 0.1 μM (absorption change) (Pelletier and Kraut, 1992) | XRD, 2PCB 2.8 Å (Pelletier and Kraut, 1992) |
| NDH-1 | Ferredoxin | Thermosynechococcus elongatus | 1 μM (SPR) (Pan et al., 2020) | EM, 6KHI 3.2 Å (Pan et al., 2020), 6L7O 3.2 Å [not yet published] |
| Japanese encephalitis virus | 2F2 Fab fragment | Mus musculus | 6.5 nM (SPR) (Qiu et al., 2018) | EM, 5YWO 4.7 Å (Qiu et al., 2018) |
| Japanese encephalitis virus | 2H4 Fab fragment | Mus musculus | 2.9 nM (SPR) (Qiu et al., 2018) | EM, 5YWP 4.6 Å (Qiu et al., 2018) |
| 70S Ribosome | YidC insertase | Escherichia coli | 0.2 μM (FCS) (Kedrov et al., 2016) | EM, 5M5H 4.5 Å (Kedrov et al., 2016) |
| RNA polymerase | Transcription termination factor | Escherichia coli | 0.31 μM (enzymatic) (Kang et al., 2017) | EM, 6ALGa 3.7 Å (Kang et al., 2017) |
| Rubisco | M35 reduced | Synechococcus elongatus PCC 7942 | 0.2 μM (turbidity assay) (Wang et al., 2019) | EM, 6HBC 2.78 Å (Wang et al., 2019) |
| Rubisco | Rca (Rubisco activase) | R. sphaeroides | 3.1 μM (enzymatic) (Mueller-Cajar et al., 2011) | EM, EMD-3701a not resolved 7.56 Å (Bhat et al., 2017) |
a
Cross-linked complex.
b
Affinity measurement with wt Ferredoxin, structure with Gallium Ferredoxin; SPR: surface plasmon resonance; FAS: flash absorption spectroscopy; ITC: isothermal titration calorimetry; FCS: fluorescence correlation spectroscopy.