Fig. 3.

CqDVP-2 and CqDVP-4 are localized in the distal lateral lobes of C. quinquefasciatus salivary glands Immunofluorescence of female C. quinquefasciatus SGs indicates specific expression of CqDVP proteins in the distal lateral lobes of these tissues. SGs were incubated with (a) anti-CqDVP-2 or (b) anti-CqDVP-4 antibodies raised in mice. (c) Glands incubated with sera from mice immunized against only Magic Mouse adjuvant were used as a control. Anti-mouse IgG Alexa Fluor 594 was used as a secondary antibody, shown in red. Actin, stained with Phalloidin 488, is shown in green, and nuclei, stained with DAPI, are in blue. Images were captured on a Leica SP8 confocal microscope at 63x. Scale bars ​= ​100 ​μm.