Fig. 6.

CqDVP-2 and CqDVP-4 bind to carbohydrates with terminally linked galactose and galactose derivatives

(a) Glycan arrays of purified recombinant CqDVP-2 and CqDVP-4 against 300 glycans identified >10 positive hits with relative fluorescence intensities (RFI) ​>

100. The 10 glycans with the greatest RFI are shown above, with the full name of each glycan in the list below. Among the top 10 scoring glycans for each protein, seven of these returned high relative scores for both CqDVP-2 and CqDVP-4. These data indicate both CqDVP proteins showed positive binding to glycans with terminally linked galactose or N-acetylgalactosamine residues, notably G0074 and G0026. The best docking scores for these ligands are shown in (Supplementary Table 3). One negative and two positive controls were included in each array. (b) Surface maps of CqDVP-2 (left) and CqDVP-4 (right) with compound G0074 shown in a binding pose with a glide score of −11.8 and −10.2, respectively. The surface of both proteins is colored according to electrostatic potential, with blue representing strongly electropositive, and red, strongly electronegative surfaces, and ligand G0074 is colored in cyan (left) and green (right). The model was generated in Chimera.