Fig. 4.

NMR relaxation data confirms the line broadening analysis. In-cell R₂ relaxation rates in blue for TTHA^pwt (A.), red for HAH1^pwt (B.) and in green SOD1^barrel (C.), compared to the relaxation rates in dilute buffer (black solid lines). For all three proteins a significant increase in relaxation rate is observed, corresponding to a retardation of rotational diffusion. For TTHA^pwt, the R₂ rate was determined also in E. coli, showing an even more pronounced retardation (grey dashed line in A.).