Table 1.

CryoEM data collection, single-particle reconstruction maps, and model statistics.

Data collection information
Data set	EcAGPase-FBP complex	EcAGPase-AMP complex
Nominal magnification	47755x	47755x
Voltage (kV)	300	300
Electron dose (e–/Å2)	45.6	44.8
Pixel size (Å)	1.047	1.047
Movie number	2975	1681
Defocus average and range (μm)	1.4 (0.2–2.6)	1.7 (0.3–5.0)
Frames	40	40

Single-particle reconstruction information
Map	EcAGPase-FBP complex			EcAGPase-AMP complex
Final number of particle images in map	297275			94769
Symmetry imposed	D2	C2	C1	D2	C2	C1
EMDB code	EMD-4754	EMD-10199	EMD-10201	EMD-4761	EMD-10203	EMD-10208
Map resolution (Å) FSC=0.143 criterion	3.05	3.16	3.24	2.95	3.09	3.26
Map sharpening B factor (Å2)	−148.1	−139.9	131.2	−116.8	−114.5	−99.9
Model information
Model	EcAGPase-FBP complex			EcAGPase-AMP complex
PDB ID	6R8B	6SHJ	6SHN	6R8U	6SHQ	6SI8
Atoms (Non-H)	26092	25974	25359	26760	26874	26590
Protein residues	1688	1664	1644	1700	1696	1700
Ligands	4	4	2	4	4	4
Bonds (RMSD)
Length (Å)	0.01	0.01	0.016	0.01	0.01	0.01
Angles (°)	1.34	1.01	1.34	1.35	1.38	1.40
Mean B-factor
Protein	153.64	163.46	94.72	134.96	136.07	88.79
Ligand	176.65	191.65	101.34	127.23	126.19	82.53
MolProbity score	2.11	2.02	1.89	2.30	2.04	1.79
Clashscore	7.93	3.54	2.21	7.17	5.36	2.14
Rotamer outliers (%)	3.12	3.58	4.81	3.32	2.28	2.45
Ramachandran plot
Favored (%)	95.71	92.90	94.47	91.43	92.06	91.31
Allowed (%)	4.05	6.98	5.41	8.57	7.94	8.33
CC (volume)	0.77	0.75	0.80	0.80	0.78	0.79
Mean CC for ligands	0.74	0.66	0.74	0.84	0.82	0.84
CC (mask)	0.77	0.75	0.81	0.80	0.79	0.79