Fig. 4.

Purified ZnT8B forms a highly stable dimer. (A) Fluorescent thermal stability assay. Plot shows dye fluorescence per ^oC increase in temperature from which the calculated T_m of ZnT8B is 57 °C. (B) Non-denaturing PAGE of ZnT8B-G₃H₆, showing the protein-detergent micelle complex with an apparent molecular weight of ~140 kDa, which corresponds to an actual molecular weight of ~80 kDa. Positions of molecular weight markers in kDa (left). (C) MALDI-MS of purified ZnT8B-G₃H₆ showing number of sample ions detected and their corresponding mass-to-charge (m/z) ratio. The ZnT8B construct appears as both a monomer of 36.117 kDa and a dimer of 72.020 kDa.