Fig. 6.

EM and single particle analysis using RELION. Shown are the 23 final particle classes used in determining the ZnT8B structure. Top row indicates number of particles in each class average (N), second row shows image of a representative raw particle belonging to class, third row shows 2D class average, bottom row displays back projections of 3D map with view corresponding to class average shown in third row. Note the close correspondence of the 2D class averages (in which symmetry was not applied) with the back projections of the 3D map (in which C2 symmetry was imposed), suggesting that ZnT8 assembles as a symmetric dimer.