Table 1.
Rates of zinc ion uptake in transformed P. pastoris cell cultures. Rates derived from linear domain of zinc uptake assay, from 1 h timepoint on. Units are in pmol/minute. Values are the average of at least three replicates and are shown with the standard error.
| MMY (unbuffered) |
BMMY |
||||
|---|---|---|---|---|---|
| pH 4.0 | pH 5.0 | pH 6.0 | pH 6.0 + Cd2+ | pH 6.0 | |
| vector only | 3.9 ± 0.7 | 6.2 ± 1.5 | 5.6 ± 0.1 | 2.7 ± 0.3 | 7.3 ± 0.6 |
| PpZnT8Bopt1 | 0.3 ± 0.2 | 0.6 ± 0.3 | 1.6 ± 0.4 | 0.4 ± 0.1 | 0.5 ± 0.2 |
| PpZnT8Bopt2 | 2.8 ± 0.5 | 4.3 ± 1.2 | 4.4 ± 1.1 | 1.4 ± 0.2 | 3.4 ± 0.5 |