Table 2.

Secondary structure fractions calculated by deconvolution of circular dichroism spectra. Shown are results for the bacterial thioredoxin-ZnT8A amino terminal domain fusion (TRX-ntZnT8A), the ZnT8A carboxy-terminal domain (ctZnT8A), the carboxy-terminal domain of YiiP (ctYiiP), and bacterial thioredoxin (TRX). Deconvolution solutions produced by the CDSSTR method, using a reference dataset of 128 membrane and soluble protein structures (Abdul-Gader et al., 2011). Secondary structure fractions noted are regular α-helix (α_R), distorted α-helix (α_D), regular β-strand (β_R), distorted β-strand (β_D), β-turn (T), and unordered (U), as defined by (Sreerama et al., 1999). The normalized root mean square deviation (NRMSD) is shown for each solution.

protein	αR	αD	βR	βD	T	U	NRMSD
TRX-ntZnT8A	0.23	0.16	0.11	0.07	0.12	0.32	0.016
ctZnT8A	0.12	0.11	0.18	0.10	0.12	0.36	0.039
ctYiiP (X-ray)a	0.33		0.26		0.19	0.23b	–
TRX	0.13	0.10	0.23	0.07	0.10	0.36	0.029
TRX (X-ray)a	0.39		0.30		0.15	0.16	–

^aSecondary structure assignment using STRIDE (Kabsch and Sander, 1983).

^b12% unassigned content and 11% disordered.