TABLE 1.
The amounts (nmol/mg protein) of the metabolites alanine, aspartate, glutamate, glutamine, and GABA in extracts from hippocampal and cortex slices from Glud1lox/lox and CNS-Glud1–/– mice was determined by high performance liquid chromatography (HPLC).
| Amounts (nmol/mg protein) of amino acids in hippocampal slice extracts | ||||||
| Brain area | Genotype | Alanine | Aspartate | Glutamate | Glutamine | GABA |
| Hippocampus | Glud1lox/lox | 1.68 ± 0.34 | 72.5 ± 8.4 | 260.6 ± 23.2 | 9.90 ± 1.45 | 21.6 ± 2.4 |
| Glud1–/– | 4.69 ± 0.66* | 69.3 ± 8.8 | 290.6 ± 29.7 | 9.00 ± 1.09 | 21.2 ± 2.3 | |
| Cortex | Glud1lox/lox | 5.10 ± 0.92 | 73.0 ± 9.2 | 268.3 ± 30.9 | 13.4 ± 1.97 | 21.6 ± 2.7 |
| Glud1–/– | 3.62 ± 0.71 | 72.0 ± 14.8 | 298.1 ± 36.5 | 8.58 ± 1.12 | 19.0 ± 2.5 | |
The results are presented as averages ± SEM based on measurement of slices from 9 to 10 individual mice. Statistically significant differences (P < 0.05) between Glud1lox/lox and CNS-Glud1–/– mice are indicated with an asterisk.