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. 2021 May 19;12:675260. doi: 10.3389/fgene.2021.675260

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Copyright © 2021 Szurman-Zubrzycka, Chwiałkowska, Niemira, Kwaśniewski, Nawrot, Gajecka, Larsen and Szarejko.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hierarchical clustering of samples using distance based on Pearson’s correlation coefficient with Ward D2 linkage algorithm for a set of genes differentially expressed in barley root meristems in the long-term experiment. Gene counts were regularized log (rlog) transformed with library size-wise normalization, scaled and centered to be represented as z-scores in the log2 scale. The heat map represents the relative expression levels of DEGs. (A) The comparison between pH = 4.0 and pH = 6.0. (B) The comparison between Al-treatment at pH = 4.0 and pH = 4.0 without Al.