Figure 4.

SIRT3 deficiency induces apoptosis of kidney tubular epithelial cells. (A) The apoptosis of kidney tissues detected by TUNEL assay. (B) Apoptotic rate of kidney in each group. (C) RT-qPCR detection of Bcl-2 mRNA in the kidney. (D) RT-qPCR detection of Bax mRNA in the kidney. (E) RT-qPCR detection of Caspase-3 mRNA in the kidney. SIRT3, Sirtuin3; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay. Compared with WT-Sham group, **P<0.01, ***P<0.001; compared with KO-Sham group, **P<0.01, ***P<0.001; compared with WT-CLP group, #P<0.05, ##P<0.01