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. 2021 Mar 22;42(18):1742–1756. doi: 10.1093/eurheartj/ehab107

Figure 2.

Figure 2

Functional effects of rs10754555 on expression of NLRP3 and inflammasome activation in freshly isolated human peripheral blood mononuclear cells. (A) Experimental work-flow. (B) mRNA expression of NLRP3 in freshly isolated peripheral blood mononuclear cells. (C) Plasma levels of interleukin-18 and (D) and interleukin-1β according to rs10754555 genotype. (E) Representative fluorescence microscopy of Alexa Fluor-488-labeled ASC specks from plasma and GFP-ASC in the supernatant of THP-1 cells (representative of three independent experiments). (F) Mean fluorescence intensity of ASC specks in plasma samples according to rs10754555 genotype. (G) Representative flow cytometry images of ASC speck quantification in plasma. Each dot represents an individual patient, and whiskers of the box plots represent 5 and 95 percentiles. LPS, lipopolysaccharide; qPCR, quantitative polymerase chain reaction; TNF, tumour necrosis factor.